A Fluorescent Gram Stain for Flow Cytometry and Epifluorescence Microscopy
نویسندگان
چکیده
منابع مشابه
A fluorescent Gram stain for flow cytometry and epifluorescence microscopy.
The fluorescent nucleic acid binding dyes hexidium iodide (HI) and SYTO 13 were used in combination as a Gram stain for unfixed organisms in suspension. HI penetrated gram-positive but not gram-negative organisms, whereas SYTO 13 penetrated both. When the dyes were used together, gram-negative organisms were rendered green fluorescent by SYTO 13; conversely, gram-positive organisms were rendere...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1998
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.64.7.2681-2685.1998